catalase prac

Research Question
This experiment investigates the enzyme called catalase.
Catalase is a very fast enzyme that breaks tear down toxic hydrogen peroxide (bleach) into harmless water and type O gas

2H2O2 2H2O+O2
This is important because catalase is found in blood, muscle, liver and many another(prenominal) tissues in the carcass. H2O2 is made in small quantities in the body and must be removed quickly before it causes damage. The sustain of this experiment is to investigate how the number of substrate submersion affects the score of inhibition of catalase by the inhibitor slovenly person sulphate.

Hypothesis
Hypothesis: The higher the substrate concentration, the less the degree of inhibition of catalase by the inhibitor copper sulphate.

debate: The higher the substrate concentration, the less chance the particles are expiration to collide, which therefore means that the less likely hood the catalase enzymes are going to inhibit the liver. This would result in there organism a lesser degree of inhibition by the inhibitor copper sulphate.





Variables
Dependent Variable: Reaction rate
Independent Variable: concentration of the substrate
Controlled Variables: The size of the cubes
The time the stop watch was started
The amount of detergent
The amount of H2O2
The volume of copper sulfate
The size of the measuring cylinder

Apparatus
5ml of copper sulfate
5ml of water
20 drops of detergent
20ml of H2O2 1.5%
20ml of H2O2 3%
20ml of H2O2 4.5%
20ml of H2O2 6%
20 1cm3 cubes of liver
Stopwatch
10 large scrutiny tubes
Test tube rack
100ml measuring cylinder
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